BIOEducation

What is BIOEducation?

Our BIOEducation program provides specific technical and business training that is tailored to the needs of our clients.

Participants can choose from a wide variety of courses on business and technical competences that are important for future success.

Participants can acquire a better understanding of the industry and gain basic and specialised knowledge in areas such as innovation, market analysis, definition of business strategies, financing, legal affairs, management and negotiation skills.

All this know-how is essential for the successful development of business projects.

Bioedukacija 1@2x Scaled

UPCOMING WORKSHOPS:

BIOCentre organises workshops, seminars and hands-on training for groups and individuals. Our experienced researchers organise training courses in collaboration with leading experts from academia and the industry.

We aim to meet our client’s specific needs, education level and schedule.

Biocentar66 Scaled

UPCOMING WORKSHOPS:

LIST OF WORKSHOPS FOR GROUPS, PROVIDED ONCE A YEAR

Laboratory

Contact Workshop topic

Duration

Bioanalytical laboratory I
(Chemistry)

Anita Šporec, PhD

anita.sporec@biocentre.hr

Introduction to gas and liquid chromatography

1st day – lectures

2nd day – laboratory exercises – GC workshop

3rd day – laboratory exercises – LC workshop

Liquid chromatography (UHPLC) – method development and validation

1st day – laboratory exercises – method development

2nd day – laboratory exercises – method validation

3rd day – laboratory exercises – data analysis

Gas chromatography method development (GC‑MS/MS)

1st day – MRM method development (liquid injection)

2nd day – Full scan method development (headspace injection)

Bioanalytical laboratory II
(Proteomics)

Ana Butorac, PhD

ana.butorac@biocentre.hr

Basics of proteomics

1st day – lectures

2nd day – laboratory exercises

Liquid chromatography and mass spectrometry

1st day – lectures

2nd day – laboratory exercises

Cell biology and Microbiology laboratory

Dora Markulin, PhD

dora.markulin@biocentre.hr

Selected methods in molecular biology (PCR, Western blot, flow cytometry, cell culture, DNA / RNA analysis, etc.)

5 working days in (lectures and exercises)

Workshops are normally announced a couple of months ahead with all the relevant information regarding the timeline and registration. Additional information is provided on our web page, BIOCentre’s Facebook page, and in our Newsletter.

 

LIST OF OUR TAILOR-MADE WORKSHOPS ORGANIZED UPON REQUEST
Workshops can be organized for an individual or for a group of participants

Laboratory

Contact Workshop topic

Instruments

Bioanalytical laboratory I
(Chemistry)

Anita Šporec, PhD

anita.sporec@biocentre.hr

Basic training for chromatography HPLC‑UV/VIS (FLD, RID), LC‑MS/MS, GC‑FID, GC‑MS/MS
Method development LC‑MS/MS, HPLC‑UV/VIS (FLD, RID), GC‑FID, GC‑MS/MS
Cleaning validation process using swabs HPLC‑UV/VIS
Gas and / or liquid chromatography Tips & Tricks HPLC‑UV/VIS (FLD, RID), GC‑FID
Validation of analytical methods UV/VIS spectrophotometer, gas chromatography, liquid chromatography
Gas chromatography method development (GC‑MS/MS) using Solid Phase Micro Fiber Extraction (SPME) GC‑MS/MS
Spectrophotometric and spectroscopic instrumental analysis in the laboratory: theoretical lectures and principles of analysis UV/VIS spectrophotometer, Spectrofluorometer, Infrared spectroscopy (FTIR)
Sample preparation for specific analysis: pharmaceutical, cosmetic, food products, etc.

Good Laboratory Practice (GLP): theoretical lectures and practical advice for safe laboratory work and reliable high-quality results

Bioanalytical laboratory II
(Proteomics)

Ana Butorac, PhD

ana.butorac@biocentre.hr

Peptide mass mapping Nano-LC and MALDI-TOF/TOF
Analysis of protein intact mass MALDI-TOF/TOF
Protein gel electrophoresis Gel electrophoresis device
In-gel and in-solution protein identification (MS based identification) Nano-LC and MALDI-TOF/TOF
Protein liquid chromatography (SEC and RP-LC) HPLC – UV/VIS
Amino acid analysis HPLC – FLD
Targeted protein analysis LC-MS/MS
Analysis of secondary and tertiary protein structure CD

Microbiology laboratory

Matea Skoblar, M.Eng

matea.skoblar@biocentre.hr

Bioreactor systems – types, components and use BIOSTAT Qplus 12 fold 0.5 L, BIOSTAT B Twin 2 L, BIOSTAT Cplus 30 L
Bacterial metabolism and fermentation principles

Process optimization for production of biologics BIOSTAT Qplus 12 fold 0.5 L
Biomass collection and extraction of biological material (sonification, high-pressure homogenization) Continous flow centrifuge, high pressure homogenizer, ultrasonic homogenizer
Bioprocess design (rates, q-rates, balances)

Effects of xenobiotics and phytochemicals on pathogens and probiotics (study design)

Basics of genetic engineering (mutagenesis, cloning and transformation)

Sterilization and disinfection procedures

Microbiological nutrient media – types and preparation

Isolation, cultivation and identification of microorganisms from different sources (water, wine, beer, environment)

Bacterial morphology and identification methods (microscopy, Gram staining, PCR) Optical microscope, Instrument for PCR and qPCR
Antibiotic sensitivity testing and interpretation

Cell biology laboratory

Dora Markulin, PhD

dora.markulin@biocentre.hr

Sample preparation and flow cytometry Flow cytometer BD FACSVerse (8 colours)
Aseptic laboratory techniques, cultivation and maintenance of human and animal cell lines, supporting cells (“Nurse-like cells”), freeze/thaw procedures, peripheral blood and bone marrow lymphocytes isolation

Cell proliferation and cytotoxicity assays (MTT)

DNA, RNA and total protein isolation from cells and tissues, ELISA test Multi detection microplate reader, UV-Vis spectrophotometer
PCR and qPCR (polymerase chain reaction) – basics, optimization and troubleshooting Instrument for PCR and qPCR
Cell line transfection using lipid reagents

Cultivation of animal and human cell lines in bioreactors. Bioreactor types, components and uses. Mammalian cell metabolism. Production of recombinant proteins in mammalian cells BIOSTAT Qplus 12 fold 0.5L, BIOSTAT B Twin 2L, BIOSTAT Cplus 30L

Downstream processing laboratory

Marina Markeš, M. Sc.

marina.markes@biocentre.hr

Preparative liquid chromatography techniques ÄKTA avant
Method development for isolation of a selected biological compound ÄKTA avant
Analytical to preparative method scale up HPLC-UV/VIS and ÄKTA avant