BIOEducation

BIOEducation

The BIOEducation program is designed to provide 2 types of training:

  1. Business training (focused on Business development)
  2. Technical training (focused on Product development)

Business development courses will provide training and support in innovation management, business strategy, market analysis, business financing, legal issues, planning and negotiation skills.

Product development courses should enable tenants and clients to receive training and support in process development, GLP (good laboratory practice) procedures and regulatory compliance.

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Professional education

Professional education provides necessary training for participants who wish to get familiarized with latest developments in selected topics, good laboratory practice (GLP) and the legal framework and compliance.
Participants will gain practical knowledge while working in sophisticated laboratories together with our teaching team, and learn about the latest analytical methods and technology solutions.

We organize and conduct our courses and seminars in collaboration with renowned universities, scientists and experts from the bioscience field. Our Professional education can be tailor-made to suit the specific need of a single participant, company or an organization.

LIST OF WORKSHOPS FOR GROUPS, PROVIDED ONCE A YEAR

Laboratory Contact Workshop topic Duration
Bioanalytical laboratory I – Chemistry

 

Anita Šporec, PhD

anita.sporec@biocentre.hr

 

Introduction to gas and liquid chromatography 1st day – lectures (all participants)
2nd day – lab-based GC workshop
3rd –   lab-based LC workshop
Liquid chromatography (UHPLC) – method development and validation Lab-based training:

1st day – method development
2nd day – method validation
3rd day – data analysis

Sample preparation and gas chromatography methods (GC-MS / MS) development Lab-based training:

1st day –  sample preparation
2nd day – method development

Bioanalytical laboratory II – Proteomics Ana Butorac, PhD

ana.butorac@biocentre.hr

Basics of proteomics 1st day – lectures
2nd day – lab-based exercises
Cell biology and Microbiology laboratory Marko Marjanović, PhD

marko.marjanovic@biocentre.hr

 

Selected methods in molecular biology (PCR, Western blot, flow cytometry, cell culture, DNA / RNA analysis, etc.) 5 working days – lectures and  lab-based exercises

Workshops are normally announced a couple of months ahead with all the relevant information regarding the timeline and registration. Additional information is provided on our web page, BIOCentre’s Facebook page, and in our Newsletter.

 

LIST OF OUR TAILOR-MADE WORKSHOPS ORGANIZED UPON REQUEST
Workshops can be organized for an individual or for a group of participants

Laboratory Contact Workshop topic Instruments
Bioanalytical laboratory I – Chemistry

 

Anita Šporec, PhD

anita.sporec@biocentre.hr

Basic training for chromatography HPLC-UV/VIS (FLD, RID), LC-MS/MS, GC-FID, GC-MS/MS
Method development LC-MS/MS, HPLC-UV/VIS (FLD, RID), GC-FID, GC-MS/MS
Cleaning validation process using swabs HPLC-UV/VIS
Gas and / or liquid chromatography Tips & Tricks HPLC-UV/VIS (FLD, RID), GC-FID
Validation of analytical methods UV/VIS spectrophotometry, gas chromatography, liquid chromatography
Gas chromatography method development (GC-MS / MS) using Solid Phase Micro Fiber Extraction (SPME) GC-MS/MS
Spectrophotometric and spectroscopic instrumental analysis in the laboratory: theoretical lectures and principles of analysis UV/VIS spectrophotometry, Fluorescence spectrophotometry, Infrared spectroscopy (FTIR)
Sample preparation for specific analysis: pharmaceutical, cosmetic, food products, etc.
Good Laboratory Practice (GLP): theoretical lectures and practical advice for safe laboratory work and reliable high-quality results
Bioanalytical laboratory II – Proteomics Ana Butorac, PhD

ana.butorac@biocentre.hr

Peptide mass mapping Nano-LC and MALDI-TOF/TOF
Analysis of protein intact mass MALDI-TOF/TOF
Protein gel electrophoresis Gel electrophoresis device
In-gel and in-solution protein identification (MS based identification) Nano-LC and MALDI-TOF/TOF
Protein liquid chromatography (SEC and RP-LC) HPLC – UV/VIS
Amino acid analysis HPLC – FLD
Targeted protein analysis LC-MS/MS
Analysis of secondary and tertiary protein structure CD
Microbiology laboratory Adriana Lepur, PhD

adriana.lepur@biocentre.hr

Bioreactor systems – types, components and use BIOSTAT Qplus 12 fold 0.5 L, BIOSTAT B Twin 2 L, BIOSTAT Cplus 30 L
Bacterial metabolism and fermentation principles
Process optimization for production of biologics BIOSTAT Qplus 12 fold 0.5 L
Biomass collection and extraction of biological material (sonification, high-pressure homogenization) Continous flow centrifuge, high pressure homogenizer, ultrasonic homogenizer
Bioprocess design (rates, q-rates, balances)
Effects of xenobiotics and phytochemicals on pathogens and probiotics (study design)
Basics of genetic engineering (mutagenesis, cloning and transformation)
Sterilization and disinfection procedures
Microbiological nutrient media – types and preparation
Isolation, cultivation and identification of microorganisms from different sources (water, wine, beer, environment)
Bacterial morphology and identification methods (microscopy, Gram staining, PCR) Optical microscope, Instrument for PCR and qPCR
Antibiotic sensitivity testing and interpretation
Cell biology laboratory Marko Marjanović, PhD

marko.marjanovic@biocentre.hr

Sample preparation and flow cytometry Flow cytometer BD FACSVerse (8 colours)
Aseptic laboratory techniques, cultivation and maintenance of human and animal cell lines, supporting cells (“Nurse-like cells”), freeze/thaw procedures, peripheral blood and bone marrow lymphocytes isolation
Cell proliferation and cytotoxicity assays (MTT)
DNA, RNA and total protein isolation from cells and tissues, ELISA test Multi detection microplate reader, UV-Vis spectrophotometer
PCR and qPCR (polymerase chain reaction) – basics, optimization and troubleshooting Instrument for PCR and qPCR
Cell line transfection using lipid reagents
Cultivation of animal and human cell lines in bioreactors. Bioreactor types, components and uses. Mammalian cell metabolism. Production of recombinant proteins in mammalian cells BIOSTAT Qplus 12 fold 0.5L, BIOSTAT B Twin 2L, BIOSTAT Cplus 30L
Downstream processing laboratory Marina Markeš, M. Sc.

marina.markes@biocentre.hr

Preparative liquid chromatography techniques ÄKTA avant
Method development for isolation of a selected biological compound ÄKTA avant
Analytical to preparative method scale up HPLC-UV/VIS and ÄKTA avant