Cell Biology lab

Flow cytometry (BD FacsVerse, FACSuite, 3 lasers, 8 fluorochromes per sample)
• Immunophenotyping
• Hematologic analysis (Leukemia and lymphoma phenotyping, leukocyte surface antigens, identification of prognostically important subgroups, Hematopoietic progenitor cell enumeration), systemic mastocytosis, Reticulocyte enumeration, Autoimmune and alloimmune disorders (anti-platelet antibodies IgG, IgM, anti-neutrophil antibodies IgG, immune complexes), feto-maternal hemorrhage quantification, erythrocyte surface antigens, assessment of leukocyte contamination of blood products (Forward and side scatter, leukocyte surface antigens), paroxysmal nocturnal hemoglobinuria
• Immunodeficiency studies, transplantation rejection
• Specific human or animal cell surface or intra-cellular markers
• Cell line validation
• Apoptosis/viability monitoring/tests
• Visualization of fluorescent proteins
• Measurement of proliferation markers
• Chemotaxis
• Actin polymerization
• DNA content analysis
• Caspase activity

Cell culture
• Human and animal cell lines (adherent or in a suspension)
• Primary cultures
• Co-cultures, 3D cultures
• Generation of Nurse-like cells or similar supportive in vitro cell models
• Cytotoxicity, cell proliferation tests (MTT, direct contact, etc.)
• Freezing/thawing and cell banking services (liquid nitrogen)

Molecular biology
• DNA, RNA and protein extractions from cell tissue, cell suspension, blood, bone marrow, etc.
• Western blotting
• PCR (ARMS, cDNA retrotranscription, etc.)
• ELISA
• Generation of lentiviral vectors
• Transient and stable transfections
• CRISPR/CAS9 genome editing system

Cell Bioreactors
Production of drug products, cytokines, growth hormones, hematopoietic growth factor, antibodies, etc. on large scale (bioreactors BIOSTAT Qplus 12 fold 0.5L CC, BIOSTAT B Twin 2L CC, BIOSTAT Cplus 30L CC)