Bioanalytical Laboratory I (chemistry)

BIOCentre’s Bioanalytical Laboratory I (chemistry) provides services of developing, optimization, transfer and validation of various analytical methods (UV/VIS and fluorescence spectroscopy, liquid chromatography UHPLC-UV/VIS, HPLC-RID, HPLC-FLD, LC-(QQQ)-MS/MS and gas chromatography GC-(Ion-trap)-MS/MS). 
 
Using HPLC equipped with UV/VIS (DAD) detector, we can develop quantitative methods for the measurement of a wide range of compounds in the field of biopharmaceuticals, food technology, agrochemicals, cosmetics or environmental science. Some examples include  biologically active ingredients, degradation products (impurities), preservatives, antioxidants, additives, water-soluble vitamins, organic acids, biomolecules (hormones, proteins, peptides, etc.).
 
Assay determination of sugars, alcohols and complex carbohydrates can be provided using HPLC - REFRACTIVE INDEX detector. 
 
Compounds with natural fluorescence (for example, polycyclic aromatic hydrocarbons) as well as fluorescent compounds produced by pre-column derivatization (bioactive peptides, traces of drugs, etc.) can be analyzed by HPLC - FLUORESCENCE detector.
 
Aroma profiling of food, drinks, beverages, flavor and fragrance can be done using manual Head-Space–Solid Phase Micro Extraction fiber and GC-MS/MS instrument.
 
In addition, GC-MS/MS qualitative (semi quantitative) and / or quantitative analysis of numerous compounds can be done using automatic split/splitless injection for example volatile organic compounds, trace levels of contaminants, esters, fatty acids, alcohols, aldehydes, terpenes, contaminants in food, essential oils (lavender, lemon oil), perfumes, menthol. 
 
GC-MS/MS analysis can be used for metabolite identification and profiling, fatty acid profiling in microbes, detection of metabolites in serum, as well detection of volatile lipophilic compounds in plant species. 
 
For targeted analysis, we can developed LC-MS/MS methods for quantification of trace organic compounds in complex matrices: food safety studies, environmental studies, drug discovery, toxicology, forensics and bioanalysis.